High Kanamycin Concentration as Another Stress Factor Additional to Temperature to Increase pDNA Production in E. coli DH5α Batch and Fed-Batch Cultures
نویسندگان
چکیده
منابع مشابه
Modeling of overflow metabolism in batch and fed-batch cultures of Escherichia coli.
A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only...
متن کاملEngineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode
BACKGROUND Plasmid DNA (pDNA) is a promising molecule for therapeutic applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) using fed-batch mode. The typical limitations of such cultivations, including metabolic deviations like aerobic acetate production due to the existence of substrate gradients in large-scale bioreactors, remain as serious challenges for...
متن کاملRECOMBINANT Erwinia carotovora L-ASPARAGINASE II PRODUCTION IN Escherichia coli FED-BATCH CULTURES
Asparaginases are the cornerstone therapy of many successful combination regimens for the treatment of acute lymphoblastic leukemia (ALL), the most common malignancy in children and adolescents. The aim of this work was to produce recombinant Erwinia carotovora L-asparaginase II in Escherichia coli fed-batch cultures. Using a robust fed-batch technique with pre-determined exponential feeding ra...
متن کاملComparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa
This paper presents a comparison between batch and three different sets of fed batch fermentations forrhamnolipid production by Pseudomonas aeruginosa. The batch run was performed with 500 ml of culturemedium having the initial glycerol and sodium nitrate concentrations of 30 and 8.3 g/l, respectively. For a fedbatch run with nitrogen source in feed, 250 ml of the nitrogen exc...
متن کاملThe Temperature-limited Fed-batch Technique for Control of Escherichia Coli Cultures
The objective of this study was to investigate the physiology and productivity in Escherichia coli cultures with emphasis on the temperature-limited fed-batch (TLFB) culture. The TLFB technique controls the oxygen consumption rate of the growing culture by a gradually declining temperature from 37-35 °C down to 20-18 °C. The temperature regulated the DOT around a set-point (30 % air sat.), and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microorganisms
سال: 2019
ISSN: 2076-2607
DOI: 10.3390/microorganisms7120711